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Reagents for a single 100 μL PCR reaction for the introduction of the poly(A)-tails

Journal: STAR Protocols

Article Title: Protocol for in vitro transcribing mRNAs with defined poly(A)-tail lengths and visualizing sequential PABP binding

doi: 10.1016/j.xpro.2024.103284

Figure Lengend Snippet: Reagents for a single 100 μL PCR reaction for the introduction of the poly(A)-tails

Article Snippet: Pfu DNA polymerase buffer (10×) , In-house made, commercial alternative from Promega , Cat#M7741.

Techniques: Concentration Assay

Reagents for a 20 μL PCR reaction for the amplification of the reverse transcribed cDNA products

Journal: STAR Protocols

Article Title: Protocol for in vitro transcribing mRNAs with defined poly(A)-tail lengths and visualizing sequential PABP binding

doi: 10.1016/j.xpro.2024.103284

Figure Lengend Snippet: Reagents for a 20 μL PCR reaction for the amplification of the reverse transcribed cDNA products

Article Snippet: Pfu DNA polymerase buffer (10×) , In-house made, commercial alternative from Promega , Cat#M7741.

Techniques: Amplification, Reverse Transcription, Concentration Assay

Journal: STAR Protocols

Article Title: Protocol for in vitro transcribing mRNAs with defined poly(A)-tail lengths and visualizing sequential PABP binding

doi: 10.1016/j.xpro.2024.103284

Figure Lengend Snippet:

Article Snippet: Pfu DNA polymerase buffer (10×) , In-house made, commercial alternative from Promega , Cat#M7741.

Techniques: Recombinant, Purification, Gel Extraction, Reverse Transcription, Ligation, Sequencing, Software

10× Pfu DNA polymerase buffer

Journal: STAR Protocols

Article Title: Protocol for in vitro transcribing mRNAs with defined poly(A)-tail lengths and visualizing sequential PABP binding

doi: 10.1016/j.xpro.2024.103284

Figure Lengend Snippet: 10× Pfu DNA polymerase buffer

Article Snippet: Pfu DNA polymerase buffer (10×) , In-house made, commercial alternative from Promega , Cat#M7741.

Techniques: Concentration Assay